Paper-Based Loop Mediated Isothermal Amplification (LAMP) Platforms: Integrating the Versatility of Paper Microfluidics with Accuracy of Nucleic Acid Amplification Tests

Paper-based diagnostics offer a promising alternative to traditional diagnostic methods for point-of-care use due their low cost, ease of use, portability, rapid results, versatility, and environmental impact. While paper-based serology tests in the form lateral flow assays can provide test results past pathogen exposure, they currently lack accuracy sensitivity offered by molecular such as polymerase chain reaction (PCR). Loop-mediated isothermal amplification (LAMP)—an nucleic acid (NAAT)—provides PCR-like performance while simultaneously reducing instrumentation assay complexity associated with PCR. In this review, we discuss newly emerging class LAMP platforms that integrates versatility paper microfluidics NAATs. Since its first adoption 2015, have discussed all terms substrates, reagent incorporation techniques, platform design, heating hardware, detection methods, specificity assays. We conclude identifying current challenges future prospects